Establishment of an optimized PCR method using sequence-specific primers for screening multiple gene polymorphisms simultaneously
نویسندگان
چکیده
منابع مشابه
Establishment of an optimized PCR method using sequence-specific primers for screening multiple gene polymorphisms simultaneously.
The present study aimed to explore the factors that affect polymerase chain reaction using sequence-specific primers (PCR-SSP) and to establish an optimized PCR-SSP method for detecting multiple gene polymorphisms simultaneously. The amplification system parameters, including the concentrations of Mg2+, dNTPs, pfu Taq, primers and control primers, were optimized using the designed PCR-SSP react...
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ژورنال
عنوان ژورنال: Molecular Medicine Reports
سال: 2012
ISSN: 1791-2997,1791-3004
DOI: 10.3892/mmr.2012.1122